ssr_primer
Primer Details for TRINITY_DN77426_c3_g1_i7 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACTTTGCGCCTGCTCTTG | ACCAGTCTTTCGAGCCATGG | Start: 2756 |
| Tm | 59.969 | 60.036 | End: 2895 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | CCAACAGTCGCAAAAGCCTC | TCATGCCACTCCAACCACTC | Start: 1027 |
| Tm | 60.04 | 59.963 | End: 1233 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
|---|
| Sequence | CCATGGCTCGAAAGACTGGT | TTGTGCCACGGACATGAGAA | Start: 2876 |
| Tm | 60.036 | 59.892 | End: 3045 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | CCATGGCTCGAAAGACTGGT | TTTGTGCCACGGACATGAGA | Start: 2876 |
| Tm | 60.036 | 59.892 | End: 3046 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | GTTCCGTATGACACCGCTCA | TCATGCCACTCCAACCACTC | Start: 1118 |
| Tm | 60.109 | 59.963 | End: 1233 |
| Size | 20 | 20 | 116 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACTTTGCGCCTGCTCTTG | ACCAGTCTTTCGAGCCATGG | Start: 2756 |
| Tm | 59.969 | 60.036 | End: 2895 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
|---|
| Sequence | CCAACAGTCGCAAAAGCCTC | TCATGCCACTCCAACCACTC | Start: 1027 |
| Tm | 60.04 | 59.963 | End: 1233 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
|---|
| Sequence | CCATGGCTCGAAAGACTGGT | TTGTGCCACGGACATGAGAA | Start: 2876 |
| Tm | 60.036 | 59.892 | End: 3045 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | CCATGGCTCGAAAGACTGGT | TTTGTGCCACGGACATGAGA | Start: 2876 |
| Tm | 60.036 | 59.892 | End: 3046 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | GTTCCGTATGACACCGCTCA | TCATGCCACTCCAACCACTC | Start: 1118 |
| Tm | 60.109 | 59.963 | End: 1233 |
| Size | 20 | 20 | 116 |
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