Primer Details for TRINITY_DN77427_c7_g1_i1 |
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| (T)12c(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACCCTTACTTTCTCCTTTTTCA | TGTGTATTAAACTTAGCCAAAGAGC | Start: 10 |
| Tm | 57.991 | 57.859 | End: 289 |
| Size | 24 | 25 | 280 |
| Primer 2 details |
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| Sequence | ACCCTTACTTTCTCCTTTTTCAACA | TGTGTATTAAACTTAGCCAAAGAGC | Start: 12 |
| Tm | 58.808 | 57.859 | End: 289 |
| Size | 25 | 25 | 278 |
| Primer 3 details |
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| Sequence | TGACCCTTACTTTCTCCTTTTTCA | TGTGTATTAAACTTAGCCAAAGAGCTT | Start: 10 |
| Tm | 57.991 | 59.553 | End: 289 |
| Size | 24 | 27 | 280 |
| Primer 4 details |
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| Sequence | GACCCTTACTTTCTCCTTTTTCAACA | TGTGTATTAAACTTAGCCAAAGAGC | Start: 11 |
| Tm | 59.68 | 57.859 | End: 289 |
| Size | 26 | 25 | 279 |
| Primer 5 details |
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| Sequence | TGACCCTTACTTTCTCCTTTTTCAAC | TGTGTATTAAACTTAGCCAAAGAGC | Start: 10 |
| Tm | 59.68 | 57.859 | End: 289 |
| Size | 26 | 25 | 280 |
