ssr_primer
Primer Details for TRINITY_DN77503_c2_g1_i1 |
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| (G)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGAAAAATGGGACGGAT | ATTTTTCACGCGGGACTTGC | Start: 346 |
| Tm | 60.033 | 60.04 | End: 494 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
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| Sequence | ACGAGGGGGAAAAATGGGAC | ATTTTTCACGCGGGACTTGC | Start: 342 |
| Tm | 59.961 | 60.04 | End: 494 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
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| Sequence | AATCTTGAGCAGCGCAAACG | ATCCGTCCCATTTTTCCCCC | Start: 151 |
| Tm | 60.11 | 60.033 | End: 365 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
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| Sequence | AATCTTGAGCAGCGCAAACG | GTCCCATTTTTCCCCCTCGT | Start: 151 |
| Tm | 60.11 | 59.961 | End: 361 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
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| Sequence | GGGGGAAAAATGGGACGGAT | GGGACTTGCGGGAGTTACTT | Start: 346 |
| Tm | 60.033 | 59.676 | End: 483 |
| Size | 20 | 20 | 138 |
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