ssr_primer
Primer Details for TRINITY_DN77533_c3_g4_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCTCATGCACACATTGAT | AAGAATCCTCCCTCCCTCCC | Start: 492 |
| Tm | 59.9 | 60.03 | End: 608 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | CCTCTGACGTAAGGGCGTTT | CGAGATGGAGTGTGAGACCG | Start: 125 |
| Tm | 60.038 | 59.9 | End: 276 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CCTCTGACGTAAGGGCGTTT | CGGACGAGATGGAGTGTGAG | Start: 125 |
| Tm | 60.038 | 59.9 | End: 280 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | CCTCTGACGTAAGGGCGTTT | GATGGAGTGTGAGACCGTGG | Start: 125 |
| Tm | 60.038 | 60.109 | End: 273 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | CCCGATCGTAGCGACAGATC | GGCTCACGCGAATATCGAGA | Start: 16 |
| Tm | 60.11 | 60.04 | End: 163 |
| Size | 20 | 20 | 148 |
(T)10actcagccgtgtcttgtcatgacggtcgttcaagtacgacgtaagccactttt(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCTCATGCACACATTGAT | AAGAATCCTCCCTCCCTCCC | Start: 492 |
| Tm | 59.9 | 60.03 | End: 608 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | CCTCTGACGTAAGGGCGTTT | CGAGATGGAGTGTGAGACCG | Start: 125 |
| Tm | 60.038 | 59.9 | End: 276 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CCTCTGACGTAAGGGCGTTT | CGGACGAGATGGAGTGTGAG | Start: 125 |
| Tm | 60.038 | 59.9 | End: 280 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | CCTCTGACGTAAGGGCGTTT | GATGGAGTGTGAGACCGTGG | Start: 125 |
| Tm | 60.038 | 60.109 | End: 273 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | CCCGATCGTAGCGACAGATC | GGCTCACGCGAATATCGAGA | Start: 16 |
| Tm | 60.11 | 60.04 | End: 163 |
| Size | 20 | 20 | 148 |
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