ssr_primer
Primer Details for TRINITY_DN77701_c3_g4_i1 |
|---|
| (T)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGGACTGGTTTCGACTGC | CGCAAAAACGCTGACACTGA | Start: 367 |
| Tm | 59.966 | 59.973 | End: 616 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | TCAGGACTGGTTTCGACTGC | TGTTCTCGGTCGATATGGCG | Start: 367 |
| Tm | 59.966 | 59.97 | End: 634 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | TCAGGACTGGTTTCGACTGC | CGGAAAGTGTTCTCGGTCGA | Start: 367 |
| Tm | 59.966 | 60.04 | End: 641 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | TTCGACTGCGGTCTGCTTAG | CGCAAAAACGCTGACACTGA | Start: 378 |
| Tm | 60.109 | 59.973 | End: 616 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | GCGTTTCTGATTGGCAGGTG | CGCAAAAACGCTGACACTGA | Start: 408 |
| Tm | 60.11 | 59.973 | End: 616 |
| Size | 20 | 20 | 209 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGGACTGGTTTCGACTGC | CGCAAAAACGCTGACACTGA | Start: 367 |
| Tm | 59.966 | 59.973 | End: 616 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | TCAGGACTGGTTTCGACTGC | TGTTCTCGGTCGATATGGCG | Start: 367 |
| Tm | 59.966 | 59.97 | End: 634 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | TCAGGACTGGTTTCGACTGC | CGGAAAGTGTTCTCGGTCGA | Start: 367 |
| Tm | 59.966 | 60.04 | End: 641 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | TTCGACTGCGGTCTGCTTAG | CGCAAAAACGCTGACACTGA | Start: 378 |
| Tm | 60.109 | 59.973 | End: 616 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | GCGTTTCTGATTGGCAGGTG | CGCAAAAACGCTGACACTGA | Start: 408 |
| Tm | 60.11 | 59.973 | End: 616 |
| Size | 20 | 20 | 209 |
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