ssr_primer
Primer Details for TRINITY_DN77718_c5_g1_i1 |
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| (A)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATCAGAAAGCGGACGGT | GGGTGTATGCCAACGGTGTA | Start: 311 |
| Tm | 59.965 | 60.036 | End: 551 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
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| Sequence | TCAGCTGGTTCGGCTTCATT | ACCGTCCGCTTTCTGATTGT | Start: 65 |
| Tm | 59.963 | 59.965 | End: 330 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
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| Sequence | ACAATCAGAAAGCGGACGGT | CCTGATGCCGTTTTTGTGGG | Start: 311 |
| Tm | 59.965 | 60.038 | End: 568 |
| Size | 20 | 20 | 258 |
| Primer 4 details |
|---|
| Sequence | TGCATTGTTCACCGCCAATG | ACCGTCCGCTTTCTGATTGT | Start: 89 |
| Tm | 60.038 | 59.965 | End: 330 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
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| Sequence | AGAAAGCGGACGGTCAGAAG | GGGTGTATGCCAACGGTGTA | Start: 317 |
| Tm | 60.038 | 60.036 | End: 551 |
| Size | 20 | 20 | 235 |
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