ssr_primer
Primer Details for TRINITY_DN77742_c3_g1_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAACTTCGTGCACAGCAA | GAGCTGCTTCACGCTGAATC | Start: 256 |
| Tm | 59.973 | 59.627 | End: 418 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
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| Sequence | TTCGAGTGGCATGATGCTCC | TTTCAAGGCCAGAAGCGGAT | Start: 2 |
| Tm | 60.463 | 59.962 | End: 104 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
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| Sequence | TCTTCGAGTGGCATGATGCT | TTTCAAGGCCAGAAGCGGAT | Start: 0 |
| Tm | 59.461 | 59.962 | End: 104 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
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| Sequence | GAGTGGCATGATGCTCCAAC | TTTCAAGGCCAGAAGCGGAT | Start: 5 |
| Tm | 59.263 | 59.962 | End: 104 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
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| Sequence | CGAGTGGCATGATGCTCCAA | TTTCAAGGCCAGAAGCGGAT | Start: 4 |
| Tm | 60.745 | 59.962 | End: 104 |
| Size | 20 | 20 | 101 |
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