ssr_primer
Primer Details for TRINITY_DN77742_c6_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACTGGGTATCGTCATGGGG | TAACATGCGGTGGCGTTTTC | Start: 5 |
| Tm | 59.893 | 59.761 | End: 142 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
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| Sequence | CGCTCACTGGGTATCGTCAT | TAACATGCGGTGGCGTTTTC | Start: 1 |
| Tm | 59.614 | 59.761 | End: 142 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | CACTGGGTATCGTCATGGGG | ACATGCGGTGGCGTTTTCTA | Start: 5 |
| Tm | 59.893 | 60.604 | End: 140 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | CACTGGGTATCGTCATGGGG | CATGCGGTGGCGTTTTCTAT | Start: 5 |
| Tm | 59.893 | 59.267 | End: 139 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CGCTCACTGGGTATCGTCAT | GACTCTCCACGTATAGGCGG | Start: 1 |
| Tm | 59.614 | 59.408 | End: 103 |
| Size | 20 | 20 | 103 |
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