ssr_primer
Primer Details for TRINITY_DN77759_c2_g1_i6 |
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| (A)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGGTCTCAGGTCTCGGAGA | TCGAGTCGGTTCAAGCAGTC | Start: 577 |
| Tm | 60.035 | 60.039 | End: 733 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
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| Sequence | TTGGGAAACTGGATCACCCG | TCGAGTCGGTTCAAGCAGTC | Start: 532 |
| Tm | 59.962 | 60.039 | End: 733 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
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| Sequence | ACTGGATCACCCGAGAGACA | TCGAGTCGGTTCAAGCAGTC | Start: 539 |
| Tm | 59.96 | 60.039 | End: 733 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
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| Sequence | TTCCAAGAGCAGGTGTGACC | TCTCCGAGACCTGAGACCTG | Start: 382 |
| Tm | 59.891 | 60.035 | End: 596 |
| Size | 20 | 20 | 215 |
| Primer 5 details |
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| Sequence | TTCCAAGAGCAGGTGTGACC | CGGGTGATCCAGTTTCCCAA | Start: 382 |
| Tm | 59.891 | 59.962 | End: 551 |
| Size | 20 | 20 | 170 |
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