Primer Details for TRINITY_DN77948_c4_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAAGGAAAGCTAGAGAGAAAAAGG | TCCATTTCTCGCTTGATCGA | Start: 90 |
| Tm | 59.85 | 57.04 | End: 191 |
| Size | 26 | 20 | 102 |
| Primer 2 details |
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| Sequence | GGAAAGGAAAGCTAGAGAGAAAAAGG | TTCCATTTCTCGCTTGATCGA | Start: 90 |
| Tm | 59.85 | 57.754 | End: 192 |
| Size | 26 | 21 | 103 |
| Primer 3 details |
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| Sequence | AGGAAAGGAAAGCTAGAGAGAAAA | TCCATTTCTCGCTTGATCGA | Start: 89 |
| Tm | 57.559 | 57.04 | End: 191 |
| Size | 24 | 20 | 103 |
| Primer 4 details |
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| Sequence | AAGGAAAGGAAAGCTAGAGAGAAA | TCCATTTCTCGCTTGATCGA | Start: 88 |
| Tm | 57.559 | 57.04 | End: 191 |
| Size | 24 | 20 | 104 |
| Primer 5 details |
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| Sequence | GGAAAGGAAAGCTAGAGAGAAAAAGG | TCCATTTCTCGCTTGATCGAT | Start: 90 |
| Tm | 59.85 | 57.541 | End: 191 |
| Size | 26 | 21 | 102 |
