ssr_primer
Primer Details for TRINITY_DN78003_c1_g5_i1 |
|---|
| (C)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACATTAGCGAACGGTGCAC | TTCTGTTAACGTCGCCGACA | Start: 42 |
| Tm | 60.179 | 59.969 | End: 238 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
|---|
| Sequence | CACATTAGCGAACGGTGCAC | CAGAAGATAGCGACGTGGCT | Start: 42 |
| Tm | 60.179 | 59.899 | End: 167 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | TCACATTAGCGAACGGTGCA | TTCTGTTAACGTCGCCGACA | Start: 41 |
| Tm | 60.32 | 59.969 | End: 238 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
|---|
| Sequence | AGCCACGTCGCTATCTTCTG | TACGCAGAAACCACGCAGAA | Start: 148 |
| Tm | 59.899 | 60.25 | End: 281 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | AGCCACGTCGCTATCTTCTG | GCAGAAACCACGCAGAAACC | Start: 148 |
| Tm | 59.899 | 60.318 | End: 278 |
| Size | 20 | 20 | 131 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACATTAGCGAACGGTGCAC | TTCTGTTAACGTCGCCGACA | Start: 42 |
| Tm | 60.179 | 59.969 | End: 238 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
|---|
| Sequence | CACATTAGCGAACGGTGCAC | CAGAAGATAGCGACGTGGCT | Start: 42 |
| Tm | 60.179 | 59.899 | End: 167 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | TCACATTAGCGAACGGTGCA | TTCTGTTAACGTCGCCGACA | Start: 41 |
| Tm | 60.32 | 59.969 | End: 238 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
|---|
| Sequence | AGCCACGTCGCTATCTTCTG | TACGCAGAAACCACGCAGAA | Start: 148 |
| Tm | 59.899 | 60.25 | End: 281 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | AGCCACGTCGCTATCTTCTG | GCAGAAACCACGCAGAAACC | Start: 148 |
| Tm | 59.899 | 60.318 | End: 278 |
| Size | 20 | 20 | 131 |
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