ssr_primer
Primer Details for TRINITY_DN78490_c1_g1_i5 |
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| (A)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATTTGCGCGTGTTTGGAG | TGCCGCCCCTATTTTCATGT | Start: 236 |
| Tm | 59.762 | 60.034 | End: 341 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
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| Sequence | GCGCGTGTTTGGAGGAAAAA | TGCCGCCCCTATTTTCATGT | Start: 242 |
| Tm | 60.249 | 60.034 | End: 341 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
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| Sequence | AGATTTGCGCGTGTTTGGAG | TTGTCGTAGCCGCAAATTGC | Start: 236 |
| Tm | 59.762 | 60.11 | End: 404 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
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| Sequence | GCGCGTGTTTGGAGGAAAAA | TTGTCGTAGCCGCAAATTGC | Start: 242 |
| Tm | 60.249 | 60.11 | End: 404 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
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| Sequence | AGATTTGCGCGTGTTTGGAG | GTGTCTTTTGTCGTAGCCGC | Start: 236 |
| Tm | 59.762 | 59.837 | End: 411 |
| Size | 20 | 20 | 176 |
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