ssr_primer
Primer Details for TRINITY_DN78544_c3_g1_i4 |
|---|
| (A)14tcgaaccagaaagataaaaccagctagaaatatttcg(C)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTGTATCTGGAGATGCG | GTTATCGCGGTGGAGAGAGG | Start: 246 |
| Tm | 59.966 | 59.969 | End: 516 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | GGGGTGTATCTGGAGATGCG | GGTTATCGCGGTGGAGAGAG | Start: 246 |
| Tm | 59.966 | 59.969 | End: 517 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | TTCCTCCTGACTTGCGCATT | GTTATCGCGGTGGAGAGAGG | Start: 412 |
| Tm | 59.963 | 59.969 | End: 516 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | TTCCTCCTGACTTGCGCATT | GGTTATCGCGGTGGAGAGAG | Start: 412 |
| Tm | 59.963 | 59.969 | End: 517 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | TTTCCTCCTGACTTGCGCAT | GTTATCGCGGTGGAGAGAGG | Start: 411 |
| Tm | 59.963 | 59.969 | End: 516 |
| Size | 20 | 20 | 106 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTGTATCTGGAGATGCG | GTTATCGCGGTGGAGAGAGG | Start: 246 |
| Tm | 59.966 | 59.969 | End: 516 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | GGGGTGTATCTGGAGATGCG | GGTTATCGCGGTGGAGAGAG | Start: 246 |
| Tm | 59.966 | 59.969 | End: 517 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | TTCCTCCTGACTTGCGCATT | GTTATCGCGGTGGAGAGAGG | Start: 412 |
| Tm | 59.963 | 59.969 | End: 516 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | TTCCTCCTGACTTGCGCATT | GGTTATCGCGGTGGAGAGAG | Start: 412 |
| Tm | 59.963 | 59.969 | End: 517 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | TTTCCTCCTGACTTGCGCAT | GTTATCGCGGTGGAGAGAGG | Start: 411 |
| Tm | 59.963 | 59.969 | End: 516 |
| Size | 20 | 20 | 106 |
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