ssr_primer
Primer Details for TRINITY_DN78722_c2_g2_i3 |
|---|
| (GCG)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTACGACTCTCGGCTTACGC | GCTTCCGAGACGACCTTGAA | Start: 72 |
| Tm | 59.902 | 60.039 | End: 276 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | TTCAAGGTCGTCTCGGAAGC | TTTTAGGGAGATCCGCGACG | Start: 257 |
| Tm | 60.039 | 59.898 | End: 425 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | TTCAAGGTCGTCTCGGAAGC | AATCGGAAAGGGCCTGTAGC | Start: 257 |
| Tm | 60.039 | 60.107 | End: 534 |
| Size | 20 | 20 | 278 |
| Primer 4 details |
|---|
| Sequence | CCCAAAGCCGCTTCTACGTA | ACCTTGACACTCGTTAGCCG | Start: 41 |
| Tm | 60.109 | 60.039 | End: 141 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | CCCAAAGCCGCTTCTACGTA | GCTTCCGAGACGACCTTGAA | Start: 41 |
| Tm | 60.109 | 60.039 | End: 276 |
| Size | 20 | 20 | 236 |
(T)26: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTACGACTCTCGGCTTACGC | GCTTCCGAGACGACCTTGAA | Start: 72 |
| Tm | 59.902 | 60.039 | End: 276 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | TTCAAGGTCGTCTCGGAAGC | TTTTAGGGAGATCCGCGACG | Start: 257 |
| Tm | 60.039 | 59.898 | End: 425 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | TTCAAGGTCGTCTCGGAAGC | AATCGGAAAGGGCCTGTAGC | Start: 257 |
| Tm | 60.039 | 60.107 | End: 534 |
| Size | 20 | 20 | 278 |
| Primer 4 details |
|---|
| Sequence | CCCAAAGCCGCTTCTACGTA | ACCTTGACACTCGTTAGCCG | Start: 41 |
| Tm | 60.109 | 60.039 | End: 141 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | CCCAAAGCCGCTTCTACGTA | GCTTCCGAGACGACCTTGAA | Start: 41 |
| Tm | 60.109 | 60.039 | End: 276 |
| Size | 20 | 20 | 236 |
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