ssr_primer
Primer Details for TRINITY_DN78722_c2_g3_i2 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATAGGTGGAGCAACGCA | TGGGCCTTGACCGGATAAAC | Start: 634 |
| Tm | 59.964 | 60.035 | End: 756 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
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| Sequence | GCTTCAGATCACAACCCCCA | TGGGCCTTGACCGGATAAAC | Start: 583 |
| Tm | 59.962 | 60.035 | End: 756 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
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| Sequence | CAGCCGACAACAACAACCTG | AGCCTGTTCCTTGCATTCCA | Start: 18 |
| Tm | 59.971 | 59.887 | End: 127 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
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| Sequence | ACAATAGGTGGAGCAACGCA | GGCCTTGACCGGATAAACGA | Start: 634 |
| Tm | 59.964 | 60.108 | End: 754 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
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| Sequence | CAGATCACAACCCCCACACA | TGGGCCTTGACCGGATAAAC | Start: 587 |
| Tm | 59.89 | 60.035 | End: 756 |
| Size | 20 | 20 | 170 |
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