ssr_primer
Primer Details for TRINITY_DN78730_c4_g2_i2 |
|---|
| (C)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCACCCTTATCGACCCCTT | TGCCTCATCGCCAATCAGTT | Start: 60 |
| Tm | 59.956 | 60.035 | End: 308 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | TTCCACCCTTATCGACCCCT | TGCCTCATCGCCAATCAGTT | Start: 59 |
| Tm | 59.956 | 60.035 | End: 308 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
|---|
| Sequence | TGATAAAGGCGGCTCAGGTG | TTGTTCTCCCGCTTCGAGAC | Start: 778 |
| Tm | 60.108 | 60.039 | End: 980 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | ACCCCACATTGTTGGCATCT | CTAGGGTGCGGCTTATCTGG | Start: 118 |
| Tm | 59.885 | 59.966 | End: 359 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | ACCCCACATTGTTGGCATCT | TGCCTCATCGCCAATCAGTT | Start: 118 |
| Tm | 59.885 | 60.035 | End: 308 |
| Size | 20 | 20 | 191 |
(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCACCCTTATCGACCCCTT | TGCCTCATCGCCAATCAGTT | Start: 60 |
| Tm | 59.956 | 60.035 | End: 308 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | TTCCACCCTTATCGACCCCT | TGCCTCATCGCCAATCAGTT | Start: 59 |
| Tm | 59.956 | 60.035 | End: 308 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
|---|
| Sequence | TGATAAAGGCGGCTCAGGTG | TTGTTCTCCCGCTTCGAGAC | Start: 778 |
| Tm | 60.108 | 60.039 | End: 980 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | ACCCCACATTGTTGGCATCT | CTAGGGTGCGGCTTATCTGG | Start: 118 |
| Tm | 59.885 | 59.966 | End: 359 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | ACCCCACATTGTTGGCATCT | TGCCTCATCGCCAATCAGTT | Start: 118 |
| Tm | 59.885 | 60.035 | End: 308 |
| Size | 20 | 20 | 191 |
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