Primer Details for TRINITY_DN78848_c2_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCTAGAAAATCTCTGAATACGAGT | TCAGGATTTCCGAGAGATAAGAAGT | Start: 4 |
| Tm | 59.962 | 59.106 | End: 106 |
| Size | 26 | 25 | 103 |
| Primer 2 details |
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| Sequence | ACTCGCTAGAAAATCTCTGAATACGA | TCAGGATTTCCGAGAGATAAGAAGT | Start: 2 |
| Tm | 59.962 | 59.106 | End: 106 |
| Size | 26 | 25 | 105 |
| Primer 3 details |
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| Sequence | TCGCTAGAAAATCTCTGAATACGAGT | GGATTTCCGAGAGATAAGAAGTCTCA | Start: 4 |
| Tm | 59.962 | 59.96 | End: 103 |
| Size | 26 | 26 | 100 |
| Primer 4 details |
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| Sequence | TCGCTAGAAAATCTCTGAATACGAGT | TCAGGATTTCCGAGAGATAAGAAGTC | Start: 4 |
| Tm | 59.962 | 59.96 | End: 106 |
| Size | 26 | 26 | 103 |
| Primer 5 details |
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| Sequence | ACTCGCTAGAAAATCTCTGAATACGA | GGATTTCCGAGAGATAAGAAGTCTCA | Start: 2 |
| Tm | 59.962 | 59.96 | End: 103 |
| Size | 26 | 26 | 102 |
