ssr_primer
Primer Details for TRINITY_DN79082_c2_g1_i2 |
|---|
| (A)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTGACGTCCGATGACAAA | GGGAATTTTGGGAGAGGGGG | Start: 560 |
| Tm | 59.969 | 60.032 | End: 691 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | AACGTGACGTCCGATGACAA | GGGAATTTTGGGAGAGGGGG | Start: 559 |
| Tm | 59.969 | 60.032 | End: 691 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | CTTGCGCGGCGGTTATTTTA | TCCAAGAGGCGTATCCATGC | Start: 45 |
| Tm | 59.903 | 59.893 | End: 309 |
| Size | 20 | 20 | 265 |
| Primer 4 details |
|---|
| Sequence | CTTGCGCGGCGGTTATTTTA | CGACAGCTCTCACTCAGACC | Start: 45 |
| Tm | 59.903 | 59.83 | End: 185 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | GGTCTGAGTGAGAGCTGTCG | TCCAAGAGGCGTATCCATGC | Start: 166 |
| Tm | 59.83 | 59.893 | End: 309 |
| Size | 20 | 20 | 144 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTGACGTCCGATGACAAA | GGGAATTTTGGGAGAGGGGG | Start: 560 |
| Tm | 59.969 | 60.032 | End: 691 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | AACGTGACGTCCGATGACAA | GGGAATTTTGGGAGAGGGGG | Start: 559 |
| Tm | 59.969 | 60.032 | End: 691 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | CTTGCGCGGCGGTTATTTTA | TCCAAGAGGCGTATCCATGC | Start: 45 |
| Tm | 59.903 | 59.893 | End: 309 |
| Size | 20 | 20 | 265 |
| Primer 4 details |
|---|
| Sequence | CTTGCGCGGCGGTTATTTTA | CGACAGCTCTCACTCAGACC | Start: 45 |
| Tm | 59.903 | 59.83 | End: 185 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | GGTCTGAGTGAGAGCTGTCG | TCCAAGAGGCGTATCCATGC | Start: 166 |
| Tm | 59.83 | 59.893 | End: 309 |
| Size | 20 | 20 | 144 |
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