ssr_primer
Primer Details for TRINITY_DN79167_c4_g2_i2 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGTCGGGGTGCTTGAATC | CGTGGCCCGGAAAAGTTTTT | Start: 381 |
| Tm | 60.036 | 59.897 | End: 488 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | AAAAACTTTTCCGGGCCACG | TTGAAATGGACGGGTCTGGG | Start: 469 |
| Tm | 59.897 | 59.962 | End: 731 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | AACCCTCCACCCTACGGTTA | TTGAAATGGACGGGTCTGGG | Start: 593 |
| Tm | 59.883 | 59.962 | End: 731 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | AAAAACTTTTCCGGGCCACG | GAAATGGACGGGTCTGGGAG | Start: 469 |
| Tm | 59.897 | 60.108 | End: 729 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | AACCCTCCACCCTACGGTTA | AAAACCCGTGTTCTCCCGTT | Start: 593 |
| Tm | 59.883 | 60.107 | End: 749 |
| Size | 20 | 20 | 157 |
(G)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGTCGGGGTGCTTGAATC | CGTGGCCCGGAAAAGTTTTT | Start: 381 |
| Tm | 60.036 | 59.897 | End: 488 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | AAAAACTTTTCCGGGCCACG | TTGAAATGGACGGGTCTGGG | Start: 469 |
| Tm | 59.897 | 59.962 | End: 731 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | AACCCTCCACCCTACGGTTA | TTGAAATGGACGGGTCTGGG | Start: 593 |
| Tm | 59.883 | 59.962 | End: 731 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | AAAAACTTTTCCGGGCCACG | GAAATGGACGGGTCTGGGAG | Start: 469 |
| Tm | 59.897 | 60.108 | End: 729 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | AACCCTCCACCCTACGGTTA | AAAACCCGTGTTCTCCCGTT | Start: 593 |
| Tm | 59.883 | 60.107 | End: 749 |
| Size | 20 | 20 | 157 |
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