Primer Details for TRINITY_DN79257_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTCTTGGCAGCAAATTCAAGA | TGTAATCTGTGGAGTTGAGAAACA | Start: 71 |
| Tm | 59.362 | 58.076 | End: 171 |
| Size | 23 | 24 | 101 |
| Primer 2 details | |||
| Sequence | ACTTCTTGGCAGCAAATTCAAGA | AGCGTATCTATTGTAATCTGTGGAGT | Start: 71 |
| Tm | 59.362 | 59.734 | End: 182 |
| Size | 23 | 26 | 112 |
| Primer 3 details | |||
| Sequence | ACTTCTTGGCAGCAAATTCAAG | TGTAATCTGTGGAGTTGAGAAACA | Start: 71 |
| Tm | 58.014 | 58.076 | End: 171 |
| Size | 22 | 24 | 101 |
| Primer 4 details | |||
| Sequence | CTTGGATTAAGCAAAACAGTAGCT | AGGCGTTAAAATGATACCCCA | Start: 10 |
| Tm | 57.74 | 57.344 | End: 133 |
| Size | 24 | 21 | 124 |
| Primer 5 details | |||
| Sequence | ACTTCTTGGCAGCAAATTCAAGA | TTGTAATCTGTGGAGTTGAGAAACA | Start: 71 |
| Tm | 59.362 | 58.647 | End: 172 |
| Size | 23 | 25 | 102 |
