ssr_primer
Primer Details for TRINITY_DN79265_c0_g2_i2 |
|---|
| (G)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGTTCCGATGGTCAAAA | GCTCCCCTAGGGTAGAGAGG | Start: 25 |
| Tm | 60.251 | 59.886 | End: 225 |
| Size | 20 | 20 | 201 |
| Primer 2 details |
|---|
| Sequence | GGGGGTTCCGATGGTCAAAA | GTAATCGAGTGAGGTGCCCC | Start: 25 |
| Tm | 60.251 | 60.179 | End: 156 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | GGGGGTTCCGATGGTCAAAA | GGTAATCGAGTGAGGTGCCC | Start: 25 |
| Tm | 60.251 | 60.179 | End: 157 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | GGGGGTTCCGATGGTCAAAA | TTCTCGCTCCCCTAGGGTAG | Start: 25 |
| Tm | 60.251 | 59.815 | End: 230 |
| Size | 20 | 20 | 206 |
| Primer 5 details |
|---|
| Sequence | GGGGGTTCCGATGGTCAAAA | CGCTCCCCTAGGGTAGAGAG | Start: 25 |
| Tm | 60.251 | 60.251 | End: 226 |
| Size | 20 | 20 | 202 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGTTCCGATGGTCAAAA | GCTCCCCTAGGGTAGAGAGG | Start: 25 |
| Tm | 60.251 | 59.886 | End: 225 |
| Size | 20 | 20 | 201 |
| Primer 2 details |
|---|
| Sequence | GGGGGTTCCGATGGTCAAAA | GTAATCGAGTGAGGTGCCCC | Start: 25 |
| Tm | 60.251 | 60.179 | End: 156 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | GGGGGTTCCGATGGTCAAAA | GGTAATCGAGTGAGGTGCCC | Start: 25 |
| Tm | 60.251 | 60.179 | End: 157 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | GGGGGTTCCGATGGTCAAAA | TTCTCGCTCCCCTAGGGTAG | Start: 25 |
| Tm | 60.251 | 59.815 | End: 230 |
| Size | 20 | 20 | 206 |
| Primer 5 details |
|---|
| Sequence | GGGGGTTCCGATGGTCAAAA | CGCTCCCCTAGGGTAGAGAG | Start: 25 |
| Tm | 60.251 | 60.251 | End: 226 |
| Size | 20 | 20 | 202 |
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