ssr_primer
Primer Details for TRINITY_DN79400_c2_g3_i2 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGGGCTCATCTCAAATTG | ATGGTAACCTCTCCGCTCCT | Start: 850 |
| Tm | 59.971 | 60.032 | End: 982 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | TGCACGCAGGGATTTGTAGT | CAATTTGAGATGAGCCCGCG | Start: 659 |
| Tm | 59.964 | 59.971 | End: 869 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | GTCGTCCTCCTGACACAAGG | ATGGTAACCTCTCCGCTCCT | Start: 779 |
| Tm | 60.038 | 60.032 | End: 982 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | GGTCCACCAAGGCCGAATTA | ACTACAAATCCCTGCGTGCA | Start: 531 |
| Tm | 60.035 | 59.964 | End: 678 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
|---|
| Sequence | TGCACGCAGGGATTTGTAGT | GCCCGCGAAAACGTAAAGTT | Start: 659 |
| Tm | 59.964 | 60.041 | End: 856 |
| Size | 20 | 20 | 198 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGGGCTCATCTCAAATTG | ATGGTAACCTCTCCGCTCCT | Start: 850 |
| Tm | 59.971 | 60.032 | End: 982 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | TGCACGCAGGGATTTGTAGT | CAATTTGAGATGAGCCCGCG | Start: 659 |
| Tm | 59.964 | 59.971 | End: 869 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | GTCGTCCTCCTGACACAAGG | ATGGTAACCTCTCCGCTCCT | Start: 779 |
| Tm | 60.038 | 60.032 | End: 982 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | GGTCCACCAAGGCCGAATTA | ACTACAAATCCCTGCGTGCA | Start: 531 |
| Tm | 60.035 | 59.964 | End: 678 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
|---|
| Sequence | TGCACGCAGGGATTTGTAGT | GCCCGCGAAAACGTAAAGTT | Start: 659 |
| Tm | 59.964 | 60.041 | End: 856 |
| Size | 20 | 20 | 198 |
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