ssr_primer
Primer Details for TRINITY_DN79516_c3_g1_i2 |
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| (A)33: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATAGCCCCTCTCTCGTCGG | TCCCCGCTCCTACTGGTTTA | Start: 1332 |
| Tm | 59.964 | 59.958 | End: 1563 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
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| Sequence | CAGGATCGTGGGATGCGTAA | GTGCTCCCCGCTCTGTTATT | Start: 1860 |
| Tm | 59.896 | 60.108 | End: 1963 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
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| Sequence | TCAATCGTGGAGGCGGAAAA | AGAAAAACATGGGCCCCGAA | Start: 449 |
| Tm | 59.964 | 60.179 | End: 561 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
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| Sequence | TCAATCGTGGAGGCGGAAAA | GAGTCGGTGTGGAGATGACG | Start: 449 |
| Tm | 59.964 | 60.179 | End: 587 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
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| Sequence | AAGCGCGAAAGTTGCTTCAG | TGCCACCACCGAATCCTTTT | Start: 2148 |
| Tm | 60.041 | 60.179 | End: 2256 |
| Size | 20 | 20 | 109 |
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