ssr_primer
Primer Details for TRINITY_DN79580_c2_g1_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCTGCGGACTTCTCGGAC | CATGCAGACCTGTTTGCGAC | Start: 469 |
| Tm | 60.039 | 60.11 | End: 597 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
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| Sequence | TTTCTGCGGACTTCTCGGAC | GTGCTCTTCCGATCTCTGCA | Start: 469 |
| Tm | 60.039 | 59.826 | End: 640 |
| Size | 20 | 20 | 172 |
| Primer 3 details |
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| Sequence | TTTCTGCGGACTTCTCGGAC | TGTGCTCTTCCGATCTCTGC | Start: 469 |
| Tm | 60.039 | 59.826 | End: 641 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
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| Sequence | TCACGTCCGACCTCTCTGAT | ACACCTCTTTCGGCGTCAAA | Start: 156 |
| Tm | 60.035 | 60.179 | End: 294 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
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| Sequence | TTTGTTGTGCCAGGATCCGT | GTCCGAGAAGTCCGCAGAAA | Start: 221 |
| Tm | 60.179 | 60.039 | End: 488 |
| Size | 20 | 20 | 268 |
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