Primer Details for TRINITY_DN79609_c5_g1_i1 | |||
|---|---|---|---|
| (A)15: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGTGAAATTAAGCGTCGAAAGT | CGCTAGCGGCCTGGTTATTA | Start: 83 |
| Tm | 59.534 | 59.967 | End: 250 |
| Size | 25 | 20 | 168 |
| Primer 2 details | |||
| Sequence | AAGTGAAATTAAGCGTCGAAAGT | CGCTAGCGGCCTGGTTATTA | Start: 85 |
| Tm | 57.331 | 59.967 | End: 250 |
| Size | 23 | 20 | 166 |
| Primer 3 details | |||
| Sequence | GAAGTGAAATTAAGCGTCGAAAGT | CGCTAGCGGCCTGGTTATTA | Start: 84 |
| Tm | 58.323 | 59.967 | End: 250 |
| Size | 24 | 20 | 167 |
| Primer 4 details | |||
| Sequence | AGAAGTGAAATTAAGCGTCGAAAG | CGCTAGCGGCCTGGTTATTA | Start: 83 |
| Tm | 58.083 | 59.967 | End: 250 |
| Size | 24 | 20 | 168 |
| Primer 5 details | |||
| Sequence | AGAAGTGAAATTAAGCGTCGAAA | CGCTAGCGGCCTGGTTATTA | Start: 83 |
| Tm | 57.082 | 59.967 | End: 250 |
| Size | 23 | 20 | 168 |
