ssr_primer
Primer Details for TRINITY_DN80321_c2_g2_i4 |
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| (A)12caaaaaaacattcaagtatgtctaaaagagaaggggagagt(A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCCCATGTTCATTCCCCG | GGTTAAAAGCTAGCGGGGGT | Start: 2720 |
| Tm | 60.035 | 60.035 | End: 2855 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
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| Sequence | CCCAACCCCATGACATGTCA | GGCTTCACGAAAGGTCTCCA | Start: 2283 |
| Tm | 59.961 | 59.965 | End: 2436 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
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| Sequence | TGGAGACCTTTCGTGAAGCC | CACAGCCATACAAGCCCGTA | Start: 2417 |
| Tm | 59.965 | 60.108 | End: 2663 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
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| Sequence | GCCGCTAGTTTCCCTTAGCA | CAGTCCAGGGTAGCTTGCTC | Start: 1017 |
| Tm | 60.108 | 60.108 | End: 1121 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
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| Sequence | TGCCGCTAGTTTCCCTTAGC | CAGTCCAGGGTAGCTTGCTC | Start: 1016 |
| Tm | 60.108 | 60.108 | End: 1121 |
| Size | 20 | 20 | 106 |
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