ssr_primer
Primer Details for TRINITY_DN80433_c6_g1_i1 |
|---|
| (G)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACAAGTTCCCACGTGGT | ACTATTCGAACACGCGGGAG | Start: 128 |
| Tm | 60.035 | 60.179 | End: 248 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | CTGAGCGCCTTCAAAATCGG | ACTATTCGAACACGCGGGAG | Start: 101 |
| Tm | 59.902 | 60.179 | End: 248 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | AACACAAGTTCCCACGTGGT | GGGAGCTCATCTTGCCTACG | Start: 128 |
| Tm | 60.035 | 60.25 | End: 233 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | AACACAAGTTCCCACGTGGT | CGGGAGCTCATCTTGCCTAC | Start: 128 |
| Tm | 60.035 | 60.25 | End: 234 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | CCACGTGGTCGAATAGTGCT | ACTATTCGAACACGCGGGAG | Start: 139 |
| Tm | 60.109 | 60.179 | End: 248 |
| Size | 20 | 20 | 110 |
(T)21: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACAAGTTCCCACGTGGT | ACTATTCGAACACGCGGGAG | Start: 128 |
| Tm | 60.035 | 60.179 | End: 248 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | CTGAGCGCCTTCAAAATCGG | ACTATTCGAACACGCGGGAG | Start: 101 |
| Tm | 59.902 | 60.179 | End: 248 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | AACACAAGTTCCCACGTGGT | GGGAGCTCATCTTGCCTACG | Start: 128 |
| Tm | 60.035 | 60.25 | End: 233 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | AACACAAGTTCCCACGTGGT | CGGGAGCTCATCTTGCCTAC | Start: 128 |
| Tm | 60.035 | 60.25 | End: 234 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | CCACGTGGTCGAATAGTGCT | ACTATTCGAACACGCGGGAG | Start: 139 |
| Tm | 60.109 | 60.179 | End: 248 |
| Size | 20 | 20 | 110 |
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