ssr_primer
Primer Details for TRINITY_DN80448_c5_g1_i2 |
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| (T)32: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTGGTCACATGCGCTTTT | GGGGTTCATTGGAGTCGGTT | Start: 23 |
| Tm | 59.968 | 59.962 | End: 256 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
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| Sequence | GGTTGGTCACATGCGCTTTT | GTTGAAGTGCAGCGGAGTTG | Start: 23 |
| Tm | 59.968 | 60.041 | End: 239 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
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| Sequence | CGCAAACTGGGGGTCATTTG | GGGGTTCATTGGAGTCGGTT | Start: 77 |
| Tm | 60.038 | 59.962 | End: 256 |
| Size | 20 | 20 | 180 |
| Primer 4 details |
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| Sequence | ATTCGCAAACTGGGGGTCAT | GGGGTTCATTGGAGTCGGTT | Start: 74 |
| Tm | 59.96 | 59.962 | End: 256 |
| Size | 20 | 20 | 183 |
| Primer 5 details |
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| Sequence | CGCAAACTGGGGGTCATTTG | GTTGAAGTGCAGCGGAGTTG | Start: 77 |
| Tm | 60.038 | 60.041 | End: 239 |
| Size | 20 | 20 | 163 |
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