ssr_primer
Primer Details for TRINITY_DN80562_c4_g2_i1 |
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| (A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTTTGCCTGCGACGATGTT | GTGTTTCGTATCTTCCGCGC | Start: 128 |
| Tm | 60.04 | 59.975 | End: 250 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
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| Sequence | AAAGCCGAACCCTGTTTTGC | GTGTTTCGTATCTTCCGCGC | Start: 115 |
| Tm | 59.896 | 59.975 | End: 250 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
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| Sequence | GCGATATTCGAACTTCGCGG | GCAGGTCCCGAATTCCAAGA | Start: 317 |
| Tm | 59.841 | 60.035 | End: 543 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
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| Sequence | GCGATATTCGAACTTCGCGG | GTAAGTGGAAGAGCTGGCGT | Start: 317 |
| Tm | 59.841 | 60.037 | End: 583 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
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| Sequence | GTTCCGGCCGAAGCTAGTAA | GTGTTTCGTATCTTCCGCGC | Start: 145 |
| Tm | 59.826 | 59.975 | End: 250 |
| Size | 20 | 20 | 106 |
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