ssr_primer
Primer Details for TRINITY_DN80679_c1_g1_i4 |
|---|
| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGACTTGTGACTCACCCTG | AAGACATGTGCCCCCTAAGC | Start: 885 |
| Tm | 59.965 | 60.034 | End: 1059 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
|---|
| Sequence | GCTTAGGGGGCACATGTCTT | GGCCGCCTATTTTGCAACAA | Start: 1040 |
| Tm | 60.034 | 60.038 | End: 1183 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | TTATGGCATCATCGAGGGCC | CCTGCTGGCTGCTTCTAGTT | Start: 2319 |
| Tm | 59.963 | 60.036 | End: 2510 |
| Size | 20 | 20 | 192 |
| Primer 4 details |
|---|
| Sequence | TTGTTGCAAAATAGGCGGCC | GAAACGGGGACAGGTAGAGC | Start: 1164 |
| Tm | 60.038 | 60.108 | End: 1415 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
|---|
| Sequence | CCAACATTTTCGACGGCTGG | GGCCCTCGATGATGCCATAA | Start: 2060 |
| Tm | 60.11 | 59.963 | End: 2338 |
| Size | 20 | 20 | 279 |
(CTG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGACTTGTGACTCACCCTG | AAGACATGTGCCCCCTAAGC | Start: 885 |
| Tm | 59.965 | 60.034 | End: 1059 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
|---|
| Sequence | GCTTAGGGGGCACATGTCTT | GGCCGCCTATTTTGCAACAA | Start: 1040 |
| Tm | 60.034 | 60.038 | End: 1183 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | TTATGGCATCATCGAGGGCC | CCTGCTGGCTGCTTCTAGTT | Start: 2319 |
| Tm | 59.963 | 60.036 | End: 2510 |
| Size | 20 | 20 | 192 |
| Primer 4 details |
|---|
| Sequence | TTGTTGCAAAATAGGCGGCC | GAAACGGGGACAGGTAGAGC | Start: 1164 |
| Tm | 60.038 | 60.108 | End: 1415 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
|---|
| Sequence | CCAACATTTTCGACGGCTGG | GGCCCTCGATGATGCCATAA | Start: 2060 |
| Tm | 60.11 | 59.963 | End: 2338 |
| Size | 20 | 20 | 279 |
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