ssr_primer
Primer Details for TRINITY_DN80720_c4_g2_i3 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGCGTTTACGGCTCTCCT | CTCTTCGCTATCACGGCAGA | Start: 400 |
| Tm | 59.966 | 59.618 | End: 507 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | TTTGCGTTTACGGCTCTCCT | TCTCTTCGCTATCACGGCAG | Start: 400 |
| Tm | 59.966 | 59.618 | End: 508 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | ATTTTCCGGTCAGACGCTCC | CACGGCAGAATGAAAGAGCG | Start: 354 |
| Tm | 60.391 | 59.904 | End: 496 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | TACGGCTCTCCTCGCTATCA | CTCTTCGCTATCACGGCAGA | Start: 408 |
| Tm | 59.892 | 59.618 | End: 507 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TACGGCTCTCCTCGCTATCA | TCTCTTCGCTATCACGGCAG | Start: 408 |
| Tm | 59.892 | 59.618 | End: 508 |
| Size | 20 | 20 | 101 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGCGTTTACGGCTCTCCT | CTCTTCGCTATCACGGCAGA | Start: 400 |
| Tm | 59.966 | 59.618 | End: 507 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | TTTGCGTTTACGGCTCTCCT | TCTCTTCGCTATCACGGCAG | Start: 400 |
| Tm | 59.966 | 59.618 | End: 508 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | ATTTTCCGGTCAGACGCTCC | CACGGCAGAATGAAAGAGCG | Start: 354 |
| Tm | 60.391 | 59.904 | End: 496 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
|---|
| Sequence | TACGGCTCTCCTCGCTATCA | CTCTTCGCTATCACGGCAGA | Start: 408 |
| Tm | 59.892 | 59.618 | End: 507 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TACGGCTCTCCTCGCTATCA | TCTCTTCGCTATCACGGCAG | Start: 408 |
| Tm | 59.892 | 59.618 | End: 508 |
| Size | 20 | 20 | 101 |
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