ssr_primer
Primer Details for TRINITY_DN81007_c1_g4_i2 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTGCGCAACTTCATAGCC | TGCAGAACGAGGAAAGTGGG | Start: 462 |
| Tm | 59.8 | 60.25 | End: 588 |
| Size | 21 | 20 | 127 |
| Primer 2 details |
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| Sequence | TGGCGCCTAAAAACTCCGAA | GGCTATGAAGTTGCGCAAAGT | Start: 239 |
| Tm | 60.25 | 59.8 | End: 482 |
| Size | 20 | 21 | 244 |
| Primer 3 details |
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| Sequence | ACTTTGCGCAACTTCATAGCC | GAACGAGGAAAGTGGGGTGA | Start: 462 |
| Tm | 59.8 | 59.605 | End: 584 |
| Size | 21 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | ACTTTGCGCAACTTCATAGCC | AACGAGGAAAGTGGGGTGAG | Start: 462 |
| Tm | 59.8 | 59.603 | End: 583 |
| Size | 21 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | ACTTTGCGCAACTTCATAGCC | AGAACGAGGAAAGTGGGGTG | Start: 462 |
| Tm | 59.8 | 59.603 | End: 585 |
| Size | 21 | 20 | 124 |
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