ssr_primer
Primer Details for TRINITY_DN81049_c0_g2_i2 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCACCGCCATTTTACCCT | TCACTTGGCGATGCAGACTT | Start: 4128 |
| Tm | 60.034 | 59.965 | End: 4324 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
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| Sequence | TTAAAACTTCGGAGGGCGCT | AGGGTAAAATGGCGGTGGAG | Start: 4024 |
| Tm | 59.964 | 60.034 | End: 4147 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
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| Sequence | GAAAGACTTGGGAGAGGGGC | ATACGGGGCAGTTGCGTTAA | Start: 1832 |
| Tm | 60.035 | 60.036 | End: 1963 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
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| Sequence | GAAAGACTTGGGAGAGGGGC | AATACGGGGCAGTTGCGTTA | Start: 1832 |
| Tm | 60.035 | 60.036 | End: 1964 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
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| Sequence | GAAAGACTTGGGAGAGGGGC | TAAATACGGGGCAGTTGCGT | Start: 1832 |
| Tm | 60.035 | 60.036 | End: 1966 |
| Size | 20 | 20 | 135 |
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