ssr_primer
Primer Details for TRINITY_DN81086_c4_g1_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGGAAAGGGTTGCGAAAA | AGTCCACACAGACGCTTCAG | Start: 140 |
| Tm | 60.106 | 59.967 | End: 346 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
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| Sequence | AAGGGGAAAGGGTTGCGAAA | AGTCCACACAGACGCTTCAG | Start: 139 |
| Tm | 60.106 | 59.967 | End: 346 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
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| Sequence | CTGAAGCGTCTGTGTGGACT | CCCTGTCTCGCTGCATTGTA | Start: 327 |
| Tm | 59.967 | 60.108 | End: 546 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
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| Sequence | AATGCGGCGACTGTGAGTTA | TTTCGCAACCCTTTCCCCTT | Start: 20 |
| Tm | 60.038 | 60.106 | End: 158 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
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| Sequence | AATGCGGCGACTGTGAGTTA | TTTTCGCAACCCTTTCCCCT | Start: 20 |
| Tm | 60.038 | 60.106 | End: 159 |
| Size | 20 | 20 | 140 |
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