ssr_primer
Primer Details for TRINITY_DN81269_c1_g1_i2 |
|---|
| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGGCTCGACTGTAGATTC | ACCGTGAATCGCCTCCATTT | Start: 9 |
| Tm | 59.969 | 60.035 | End: 188 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
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| Sequence | TTACGGGGCTCGACTGTAGA | ACCGTGAATCGCCTCCATTT | Start: 6 |
| Tm | 60.035 | 60.035 | End: 188 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | CTTTTACGGGGCTCGACTGT | ACCGTGAATCGCCTCCATTT | Start: 3 |
| Tm | 60.038 | 60.035 | End: 188 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | CGGGGCTCGACTGTAGATTC | TCCACACCTACTCTCACCGT | Start: 9 |
| Tm | 59.969 | 59.888 | End: 203 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
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| Sequence | TCCCGACAAAACCTTGGAGG | AGCTGGTTTGAGCCCTGATC | Start: 1182 |
| Tm | 59.89 | 60.035 | End: 1295 |
| Size | 20 | 20 | 114 |
(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGGCTCGACTGTAGATTC | ACCGTGAATCGCCTCCATTT | Start: 9 |
| Tm | 59.969 | 60.035 | End: 188 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | TTACGGGGCTCGACTGTAGA | ACCGTGAATCGCCTCCATTT | Start: 6 |
| Tm | 60.035 | 60.035 | End: 188 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | CTTTTACGGGGCTCGACTGT | ACCGTGAATCGCCTCCATTT | Start: 3 |
| Tm | 60.038 | 60.035 | End: 188 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | CGGGGCTCGACTGTAGATTC | TCCACACCTACTCTCACCGT | Start: 9 |
| Tm | 59.969 | 59.888 | End: 203 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
|---|
| Sequence | TCCCGACAAAACCTTGGAGG | AGCTGGTTTGAGCCCTGATC | Start: 1182 |
| Tm | 59.89 | 60.035 | End: 1295 |
| Size | 20 | 20 | 114 |
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