ssr_primer
Primer Details for TRINITY_DN81428_c2_g5_i1 |
|---|
| (A)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGCAATGACAGCTTTCCCG | CCGCGCATTCACGGTTTATT | Start: 388 |
| Tm | 60.11 | 59.902 | End: 540 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | GTGCAATGACAGCTTTCCCG | ATTTTTCGTTGCGGCAACCA | Start: 388 |
| Tm | 60.11 | 59.897 | End: 503 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | AGGATTTCCTCTGGTTGCCG | TTTCAGCCCGAGATGACCTG | Start: 473 |
| Tm | 60.035 | 59.75 | End: 623 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | GGTGCAATGACAGCTTTCCC | CCGCGCATTCACGGTTTATT | Start: 387 |
| Tm | 59.755 | 59.902 | End: 540 |
| Size | 20 | 20 | 154 |
| Primer 5 details |
|---|
| Sequence | GGTGCAATGACAGCTTTCCC | ATTTTTCGTTGCGGCAACCA | Start: 387 |
| Tm | 59.755 | 59.897 | End: 503 |
| Size | 20 | 20 | 117 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGCAATGACAGCTTTCCCG | CCGCGCATTCACGGTTTATT | Start: 388 |
| Tm | 60.11 | 59.902 | End: 540 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | GTGCAATGACAGCTTTCCCG | ATTTTTCGTTGCGGCAACCA | Start: 388 |
| Tm | 60.11 | 59.897 | End: 503 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | AGGATTTCCTCTGGTTGCCG | TTTCAGCCCGAGATGACCTG | Start: 473 |
| Tm | 60.035 | 59.75 | End: 623 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | GGTGCAATGACAGCTTTCCC | CCGCGCATTCACGGTTTATT | Start: 387 |
| Tm | 59.755 | 59.902 | End: 540 |
| Size | 20 | 20 | 154 |
| Primer 5 details |
|---|
| Sequence | GGTGCAATGACAGCTTTCCC | ATTTTTCGTTGCGGCAACCA | Start: 387 |
| Tm | 59.755 | 59.897 | End: 503 |
| Size | 20 | 20 | 117 |
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