ssr_primer
Primer Details for TRINITY_DN81430_c4_g1_i3 |
|---|
| (A)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGATGCCCCTCTCTCATGA | CATCGCCGCTGTAGCATTTG | Start: 167 |
| Tm | 60.031 | 60.316 | End: 325 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
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| Sequence | ATCTCTGACTACGCTGCTGC | CATCGCCGCTGTAGCATTTG | Start: 221 |
| Tm | 59.898 | 60.316 | End: 325 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
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| Sequence | ACCTATGGATGCCCCTCTCT | AGAGCATACGCGGTTCTAGC | Start: 162 |
| Tm | 59.43 | 59.969 | End: 262 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
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| Sequence | TGGATGCCCCTCTCTCATGA | CATTTGCGTCGTGGAAAGGT | Start: 167 |
| Tm | 60.031 | 59.409 | End: 311 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
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| Sequence | GCTCTACCTATGGATGCCCC | AGAGCATACGCGGTTCTAGC | Start: 157 |
| Tm | 59.384 | 59.969 | End: 262 |
| Size | 20 | 20 | 106 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGATGCCCCTCTCTCATGA | CATCGCCGCTGTAGCATTTG | Start: 167 |
| Tm | 60.031 | 60.316 | End: 325 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
|---|
| Sequence | ATCTCTGACTACGCTGCTGC | CATCGCCGCTGTAGCATTTG | Start: 221 |
| Tm | 59.898 | 60.316 | End: 325 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | ACCTATGGATGCCCCTCTCT | AGAGCATACGCGGTTCTAGC | Start: 162 |
| Tm | 59.43 | 59.969 | End: 262 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | TGGATGCCCCTCTCTCATGA | CATTTGCGTCGTGGAAAGGT | Start: 167 |
| Tm | 60.031 | 59.409 | End: 311 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | GCTCTACCTATGGATGCCCC | AGAGCATACGCGGTTCTAGC | Start: 157 |
| Tm | 59.384 | 59.969 | End: 262 |
| Size | 20 | 20 | 106 |
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