ssr_primer
Primer Details for TRINITY_DN81881_c4_g2_i3 |
|---|
| (T)16atgtattggcacctattggaaaccggggagctgaacttttccaac(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCACAGCCACATGAAGAG | TTTGTGACTCCATGACGGGG | Start: 1483 |
| Tm | 59.963 | 59.964 | End: 1705 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | CCCCGTCATGGAGTCACAAA | TGAGAGAAGGAGCGGAGACA | Start: 1686 |
| Tm | 59.964 | 59.962 | End: 1789 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACTGTTTCCAGAGGGCAC | TTTCCACTTTGCCCCGCTAT | Start: 683 |
| Tm | 59.891 | 59.961 | End: 927 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | CACTGTTTCCAGAGGGCACT | TTTCCACTTTGCCCCGCTAT | Start: 684 |
| Tm | 59.891 | 59.961 | End: 927 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | TGAACCCACAGCCACATGAA | TTTGTGACTCCATGACGGGG | Start: 1480 |
| Tm | 59.815 | 59.964 | End: 1705 |
| Size | 20 | 20 | 226 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCACAGCCACATGAAGAG | TTTGTGACTCCATGACGGGG | Start: 1483 |
| Tm | 59.963 | 59.964 | End: 1705 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | CCCCGTCATGGAGTCACAAA | TGAGAGAAGGAGCGGAGACA | Start: 1686 |
| Tm | 59.964 | 59.962 | End: 1789 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACTGTTTCCAGAGGGCAC | TTTCCACTTTGCCCCGCTAT | Start: 683 |
| Tm | 59.891 | 59.961 | End: 927 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | CACTGTTTCCAGAGGGCACT | TTTCCACTTTGCCCCGCTAT | Start: 684 |
| Tm | 59.891 | 59.961 | End: 927 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | TGAACCCACAGCCACATGAA | TTTGTGACTCCATGACGGGG | Start: 1480 |
| Tm | 59.815 | 59.964 | End: 1705 |
| Size | 20 | 20 | 226 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCACAGCCACATGAAGAG | TTTGTGACTCCATGACGGGG | Start: 1483 |
| Tm | 59.963 | 59.964 | End: 1705 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | CCCCGTCATGGAGTCACAAA | TGAGAGAAGGAGCGGAGACA | Start: 1686 |
| Tm | 59.964 | 59.962 | End: 1789 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACTGTTTCCAGAGGGCAC | TTTCCACTTTGCCCCGCTAT | Start: 683 |
| Tm | 59.891 | 59.961 | End: 927 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | CACTGTTTCCAGAGGGCACT | TTTCCACTTTGCCCCGCTAT | Start: 684 |
| Tm | 59.891 | 59.961 | End: 927 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | TGAACCCACAGCCACATGAA | TTTGTGACTCCATGACGGGG | Start: 1480 |
| Tm | 59.815 | 59.964 | End: 1705 |
| Size | 20 | 20 | 226 |
(C)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCACAGCCACATGAAGAG | TTTGTGACTCCATGACGGGG | Start: 1483 |
| Tm | 59.963 | 59.964 | End: 1705 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | CCCCGTCATGGAGTCACAAA | TGAGAGAAGGAGCGGAGACA | Start: 1686 |
| Tm | 59.964 | 59.962 | End: 1789 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACTGTTTCCAGAGGGCAC | TTTCCACTTTGCCCCGCTAT | Start: 683 |
| Tm | 59.891 | 59.961 | End: 927 |
| Size | 20 | 20 | 245 |
| Primer 4 details |
|---|
| Sequence | CACTGTTTCCAGAGGGCACT | TTTCCACTTTGCCCCGCTAT | Start: 684 |
| Tm | 59.891 | 59.961 | End: 927 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | TGAACCCACAGCCACATGAA | TTTGTGACTCCATGACGGGG | Start: 1480 |
| Tm | 59.815 | 59.964 | End: 1705 |
| Size | 20 | 20 | 226 |
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