ssr_primer
Primer Details for TRINITY_DN82005_c4_g1_i2 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAATTTTAGCCCGCCGCAG | GCATGGTCACTCGCTTGAAC | Start: 32 |
| Tm | 60.109 | 59.833 | End: 169 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
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| Sequence | GCCGCAGCGATATCAATCAC | GCATGGTCACTCGCTTGAAC | Start: 45 |
| Tm | 59.835 | 59.833 | End: 169 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
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| Sequence | AAAATTTTAGCCCGCCGCAG | CAGGTGCGTTCATGCCAATT | Start: 32 |
| Tm | 60.109 | 59.756 | End: 279 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
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| Sequence | TCCGCGAAAGAAGCTCTTGA | CTCCGAGTTGAGAACACCCC | Start: 694 |
| Tm | 59.683 | 60.037 | End: 843 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | TCCGCGAAAGAAGCTCTTGA | CCTCCGAGTTGAGAACACCC | Start: 694 |
| Tm | 59.683 | 60.037 | End: 844 |
| Size | 20 | 20 | 151 |
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