ssr_primer
Primer Details for TRINITY_DN82036_c2_g2_i3 |
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| (T)11ctgaaatgacacgaacaac(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCTGCACCGACAACAAGT | GGGCAGTGGATACTTGGAGG | Start: 2482 |
| Tm | 60.107 | 59.819 | End: 2741 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
|---|
| Sequence | TCATTCCTGCACCGACAACA | GGGCAGTGGATACTTGGAGG | Start: 2479 |
| Tm | 59.892 | 59.819 | End: 2741 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
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| Sequence | TCTTTTGAGGTGCAGTGCCT | ACTTCCAAGATGCACTGCCA | Start: 589 |
| Tm | 59.817 | 59.889 | End: 798 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
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| Sequence | AGCCCCCTCCACCAATTTTT | ACTTCCAAGATGCACTGCCA | Start: 544 |
| Tm | 59.807 | 59.889 | End: 798 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
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| Sequence | TGCTTTGACCACAAGAGCCA | CAGCCTTCACCACCGATACA | Start: 175 |
| Tm | 60.107 | 59.751 | End: 441 |
| Size | 20 | 20 | 267 |
(T)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCTGCACCGACAACAAGT | GGGCAGTGGATACTTGGAGG | Start: 2482 |
| Tm | 60.107 | 59.819 | End: 2741 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
|---|
| Sequence | TCATTCCTGCACCGACAACA | GGGCAGTGGATACTTGGAGG | Start: 2479 |
| Tm | 59.892 | 59.819 | End: 2741 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | TCTTTTGAGGTGCAGTGCCT | ACTTCCAAGATGCACTGCCA | Start: 589 |
| Tm | 59.817 | 59.889 | End: 798 |
| Size | 20 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | AGCCCCCTCCACCAATTTTT | ACTTCCAAGATGCACTGCCA | Start: 544 |
| Tm | 59.807 | 59.889 | End: 798 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | TGCTTTGACCACAAGAGCCA | CAGCCTTCACCACCGATACA | Start: 175 |
| Tm | 60.107 | 59.751 | End: 441 |
| Size | 20 | 20 | 267 |
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