ssr_primer
Primer Details for TRINITY_DN82255_c8_g1_i2 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCACCGCGGATACTCTAAA | CGGCGGCGGTTATTTTTCTT | Start: 406 |
| Tm | 59.896 | 59.831 | End: 679 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
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| Sequence | AAGCGACGGGGAAGAAAAGT | CGGCGGCGGTTATTTTTCTT | Start: 464 |
| Tm | 59.892 | 59.831 | End: 679 |
| Size | 20 | 20 | 216 |
| Primer 3 details |
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| Sequence | GGCATTATTAAGCGACGGGG | CGGCGGCGGTTATTTTTCTT | Start: 455 |
| Tm | 59.128 | 59.831 | End: 679 |
| Size | 20 | 20 | 225 |
| Primer 4 details |
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| Sequence | AGCGACGGGGAAGAAAAGTA | CGGCGGCGGTTATTTTTCTT | Start: 465 |
| Tm | 59.029 | 59.831 | End: 679 |
| Size | 20 | 20 | 215 |
| Primer 5 details |
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| Sequence | GCCACCGCGGATACTCTAAA | CGGCGGCGGTTATTTTTCTTT | Start: 406 |
| Tm | 59.896 | 60.402 | End: 679 |
| Size | 20 | 21 | 274 |
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