ssr_primer
Primer Details for TRINITY_DN82333_c3_g3_i1 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACACCGCAAGAGGAGAAGA | GCCCAACAACCTCGAGTGAT | Start: 480 |
| Tm | 59.682 | 60.322 | End: 584 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
|---|
| Sequence | GGGACGAAGAGCTATCAGCA | ACAACTACTGTCGCCAGAGC | Start: 728 |
| Tm | 59.253 | 60.038 | End: 973 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | GGACGAGGGACGAAGAGCTA | ACAACTACTGTCGCCAGAGC | Start: 722 |
| Tm | 60.747 | 60.038 | End: 973 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | CACACCGCAAGAGGAGAAGA | GTGAAATTCTGTGCCGCAGT | Start: 480 |
| Tm | 59.682 | 59.407 | End: 614 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GAGGGACGAAGAGCTATCAGC | ACAACTACTGTCGCCAGAGC | Start: 726 |
| Tm | 60.001 | 60.038 | End: 973 |
| Size | 21 | 20 | 248 |
(TTAT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACACCGCAAGAGGAGAAGA | GCCCAACAACCTCGAGTGAT | Start: 480 |
| Tm | 59.682 | 60.322 | End: 584 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
|---|
| Sequence | GGGACGAAGAGCTATCAGCA | ACAACTACTGTCGCCAGAGC | Start: 728 |
| Tm | 59.253 | 60.038 | End: 973 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
|---|
| Sequence | GGACGAGGGACGAAGAGCTA | ACAACTACTGTCGCCAGAGC | Start: 722 |
| Tm | 60.747 | 60.038 | End: 973 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | CACACCGCAAGAGGAGAAGA | GTGAAATTCTGTGCCGCAGT | Start: 480 |
| Tm | 59.682 | 59.407 | End: 614 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GAGGGACGAAGAGCTATCAGC | ACAACTACTGTCGCCAGAGC | Start: 726 |
| Tm | 60.001 | 60.038 | End: 973 |
| Size | 21 | 20 | 248 |
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