ssr_primer
Primer Details for TRINITY_DN82381_c2_g1_i7 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCAGCTTGCACGAGATAAC | AAATCACAGGCATCTGGGGG | Start: 1060 |
| Tm | 59.974 | 60.033 | End: 1208 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
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| Sequence | AGGCGCACGAATTGTAAGGA | GCAGCGGAGGAAGCTTTTTC | Start: 1554 |
| Tm | 60.037 | 60.11 | End: 1768 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
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| Sequence | GAGACACGCAGCCTGTTAGT | CGTCCTCTGGAAGCTGTCAG | Start: 425 |
| Tm | 60.038 | 60.109 | End: 560 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
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| Sequence | CAGGCGCACGAATTGTAAGG | GCAGCGGAGGAAGCTTTTTC | Start: 1553 |
| Tm | 59.903 | 60.11 | End: 1768 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
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| Sequence | GAGACACGCAGCCTGTTAGT | CTCGTCCTCTGGAAGCTGTC | Start: 425 |
| Tm | 60.038 | 59.828 | End: 562 |
| Size | 20 | 20 | 138 |
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