ssr_primer
Primer Details for TRINITY_DN82408_c1_g1_i6 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGAGGAAGTTGCTGACC | GCCAGGAACACGTGGTAGAA | Start: 3185 |
| Tm | 59.966 | 59.966 | End: 3347 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | TGACGAGGAAGTTGCTGACC | ATGGTTTGTCCCGCATGAGT | Start: 3185 |
| Tm | 59.966 | 59.962 | End: 3307 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | ATGGTGTTTTTGCCGGAAGC | CACCGAGCGAGACCCATATC | Start: 851 |
| Tm | 59.967 | 60.039 | End: 992 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | GATATGGGTCTCGCTCGGTG | TTGTCGGAAAGAAGAGCCCC | Start: 973 |
| Tm | 60.039 | 59.964 | End: 1194 |
| Size | 20 | 20 | 222 |
| Primer 5 details |
|---|
| Sequence | CCACGAGATGAGTGACGAGG | GCCAGGAACACGTGGTAGAA | Start: 3173 |
| Tm | 59.9 | 59.966 | End: 3347 |
| Size | 20 | 20 | 175 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGAGGAAGTTGCTGACC | GCCAGGAACACGTGGTAGAA | Start: 3185 |
| Tm | 59.966 | 59.966 | End: 3347 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | TGACGAGGAAGTTGCTGACC | ATGGTTTGTCCCGCATGAGT | Start: 3185 |
| Tm | 59.966 | 59.962 | End: 3307 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | ATGGTGTTTTTGCCGGAAGC | CACCGAGCGAGACCCATATC | Start: 851 |
| Tm | 59.967 | 60.039 | End: 992 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | GATATGGGTCTCGCTCGGTG | TTGTCGGAAAGAAGAGCCCC | Start: 973 |
| Tm | 60.039 | 59.964 | End: 1194 |
| Size | 20 | 20 | 222 |
| Primer 5 details |
|---|
| Sequence | CCACGAGATGAGTGACGAGG | GCCAGGAACACGTGGTAGAA | Start: 3173 |
| Tm | 59.9 | 59.966 | End: 3347 |
| Size | 20 | 20 | 175 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGAGGAAGTTGCTGACC | GCCAGGAACACGTGGTAGAA | Start: 3185 |
| Tm | 59.966 | 59.966 | End: 3347 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | TGACGAGGAAGTTGCTGACC | ATGGTTTGTCCCGCATGAGT | Start: 3185 |
| Tm | 59.966 | 59.962 | End: 3307 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | ATGGTGTTTTTGCCGGAAGC | CACCGAGCGAGACCCATATC | Start: 851 |
| Tm | 59.967 | 60.039 | End: 992 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | GATATGGGTCTCGCTCGGTG | TTGTCGGAAAGAAGAGCCCC | Start: 973 |
| Tm | 60.039 | 59.964 | End: 1194 |
| Size | 20 | 20 | 222 |
| Primer 5 details |
|---|
| Sequence | CCACGAGATGAGTGACGAGG | GCCAGGAACACGTGGTAGAA | Start: 3173 |
| Tm | 59.9 | 59.966 | End: 3347 |
| Size | 20 | 20 | 175 |
(T)13cttttttcaagatgaacatttttcttctccatatgaagaattgatccatgaaaatttcaagcaacagcattggtttgttctcttttgat(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGAGGAAGTTGCTGACC | GCCAGGAACACGTGGTAGAA | Start: 3185 |
| Tm | 59.966 | 59.966 | End: 3347 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | TGACGAGGAAGTTGCTGACC | ATGGTTTGTCCCGCATGAGT | Start: 3185 |
| Tm | 59.966 | 59.962 | End: 3307 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | ATGGTGTTTTTGCCGGAAGC | CACCGAGCGAGACCCATATC | Start: 851 |
| Tm | 59.967 | 60.039 | End: 992 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | GATATGGGTCTCGCTCGGTG | TTGTCGGAAAGAAGAGCCCC | Start: 973 |
| Tm | 60.039 | 59.964 | End: 1194 |
| Size | 20 | 20 | 222 |
| Primer 5 details |
|---|
| Sequence | CCACGAGATGAGTGACGAGG | GCCAGGAACACGTGGTAGAA | Start: 3173 |
| Tm | 59.9 | 59.966 | End: 3347 |
| Size | 20 | 20 | 175 |
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