Primer Details for TRINITY_DN82449_c2_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAACATTCGGGAACTGCCA | TGTTCGCAATTTGATCTAAAACGT | Start: 16 |
| Tm | 59.89 | 58.246 | End: 132 |
| Size | 20 | 24 | 117 |
| Primer 2 details | |||
| Sequence | ACTGCCAAATTGCCTCTGGA | TGTTCGCAATTTGATCTAAAACGT | Start: 29 |
| Tm | 59.887 | 58.246 | End: 132 |
| Size | 20 | 24 | 104 |
| Primer 3 details | |||
| Sequence | GGGAACTGCCAAATTGCCTC | TGTTCGCAATTTGATCTAAAACGT | Start: 25 |
| Tm | 59.753 | 58.246 | End: 132 |
| Size | 20 | 24 | 108 |
| Primer 4 details | |||
| Sequence | TTCGGGAACTGCCAAATTGC | TGTTCGCAATTTGATCTAAAACGT | Start: 22 |
| Tm | 59.683 | 58.246 | End: 132 |
| Size | 20 | 24 | 111 |
| Primer 5 details | |||
| Sequence | TGAACATTCGGGAACTGCCA | TGTTCGCAATTTGATCTAAAACGTT | Start: 16 |
| Tm | 59.89 | 58.797 | End: 132 |
| Size | 20 | 25 | 117 |
