ssr_primer
Primer Details for TRINITY_DN82921_c2_g1_i5 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGATTTTGTCGACCGGCA | GAGGCAAACTCTCTCCCACG | Start: 112 |
| Tm | 59.966 | 60.39 | End: 211 |
| Size | 20 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | CGTGGGAGAGAGTTTGCCTC | TCTGCCTCAGATCACCGACT | Start: 192 |
| Tm | 60.39 | 60.324 | End: 444 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | CGTGGGAGAGAGTTTGCCTC | TTCTGCCTCAGATCACCGAC | Start: 192 |
| Tm | 60.39 | 59.467 | End: 445 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | CGTGGGAGAGAGTTTGCCTC | CTGCCTCAGATCACCGACTT | Start: 192 |
| Tm | 60.39 | 59.466 | End: 443 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
|---|
| Sequence | CTGCCGTGGGAGAGAGTTTG | TCTGCCTCAGATCACCGACT | Start: 188 |
| Tm | 60.672 | 60.324 | End: 444 |
| Size | 20 | 20 | 257 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGATTTTGTCGACCGGCA | GAGGCAAACTCTCTCCCACG | Start: 112 |
| Tm | 59.966 | 60.39 | End: 211 |
| Size | 20 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | CGTGGGAGAGAGTTTGCCTC | TCTGCCTCAGATCACCGACT | Start: 192 |
| Tm | 60.39 | 60.324 | End: 444 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | CGTGGGAGAGAGTTTGCCTC | TTCTGCCTCAGATCACCGAC | Start: 192 |
| Tm | 60.39 | 59.467 | End: 445 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | CGTGGGAGAGAGTTTGCCTC | CTGCCTCAGATCACCGACTT | Start: 192 |
| Tm | 60.39 | 59.466 | End: 443 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
|---|
| Sequence | CTGCCGTGGGAGAGAGTTTG | TCTGCCTCAGATCACCGACT | Start: 188 |
| Tm | 60.672 | 60.324 | End: 444 |
| Size | 20 | 20 | 257 |
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