ssr_primer
Primer Details for TRINITY_DN82977_c5_g3_i2 |
|---|
| (G)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTAAAGCTGGTTTGACCGC | GTGCTTCCTTCCCACAGTCA | Start: 475 |
| Tm | 59.76 | 59.891 | End: 699 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
|---|
| Sequence | TTGACCGCAAAACGTACACG | GTGCTTCCTTCCCACAGTCA | Start: 487 |
| Tm | 59.699 | 59.891 | End: 699 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | AGAACCGTCTTGGCATTCGA | AATGCCCCTGTCCATCTTCC | Start: 2033 |
| Tm | 59.68 | 59.74 | End: 2146 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | GTGGAGAACCGTCTTGGCAT | AATGCCCCTGTCCATCTTCC | Start: 2029 |
| Tm | 60.322 | 59.74 | End: 2146 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | GGTAAAGCTGGTTTGACCGC | AGTGCTTCCTTCCCACAGTC | Start: 475 |
| Tm | 59.76 | 59.602 | End: 700 |
| Size | 20 | 20 | 226 |
(A)12cattattgtgggc(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTAAAGCTGGTTTGACCGC | GTGCTTCCTTCCCACAGTCA | Start: 475 |
| Tm | 59.76 | 59.891 | End: 699 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
|---|
| Sequence | TTGACCGCAAAACGTACACG | GTGCTTCCTTCCCACAGTCA | Start: 487 |
| Tm | 59.699 | 59.891 | End: 699 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | AGAACCGTCTTGGCATTCGA | AATGCCCCTGTCCATCTTCC | Start: 2033 |
| Tm | 59.68 | 59.74 | End: 2146 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | GTGGAGAACCGTCTTGGCAT | AATGCCCCTGTCCATCTTCC | Start: 2029 |
| Tm | 60.322 | 59.74 | End: 2146 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | GGTAAAGCTGGTTTGACCGC | AGTGCTTCCTTCCCACAGTC | Start: 475 |
| Tm | 59.76 | 59.602 | End: 700 |
| Size | 20 | 20 | 226 |
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