ssr_primer
Primer Details for TRINITY_DN83201_c1_g2_i6 |
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| (C)28acaataacaaaaaacaccgaagaac(A)30: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCGTCGCAATGAAAAACT | CAGGAGGGGCACTGTGAAAT | Start: 57 |
| Tm | 60.04 | 59.961 | End: 195 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
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| Sequence | CTGGCCGTCGCAATGAAAAA | CAGGAGGGGCACTGTGAAAT | Start: 55 |
| Tm | 60.04 | 59.961 | End: 195 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
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| Sequence | AGGCGGCCCGATTATTGAAA | GATGGAGAAGGGAGAGGGGT | Start: 370 |
| Tm | 60.107 | 60.031 | End: 530 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
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| Sequence | AGGCGGCCCGATTATTGAAA | TGATGGAGAAGGGAGAGGGG | Start: 370 |
| Tm | 60.107 | 60.031 | End: 531 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
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| Sequence | ATTTCACAGTGCCCCTCCTG | TTTCAATAATCGGGCCGCCT | Start: 176 |
| Tm | 59.961 | 60.107 | End: 389 |
| Size | 20 | 20 | 214 |
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