ssr_primer
Primer Details for TRINITY_DN83289_c1_g3_i1 |
|---|
| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATGTCGGTGCGGATGAAA | TCGTGTCCGTTTGAGACCTG | Start: 560 |
| Tm | 60.037 | 59.968 | End: 747 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
|---|
| Sequence | TGACGGCCCTACTCCATGTA | GAAACCTCCCTCAGTGCCTC | Start: 706 |
| Tm | 60.033 | 60.036 | End: 826 |
| Size | 20 | 20 | 121 |
| Primer 3 details |
|---|
| Sequence | TTGTCTTGTTGGGGGCTGTT | TTTCATCCGCACCGACATGA | Start: 376 |
| Tm | 60.033 | 60.037 | End: 579 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TCATGTCGGTGCGGATGAAA | TACATGGAGTAGGGCCGTCA | Start: 560 |
| Tm | 60.037 | 60.033 | End: 725 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | AGTGTCAGGTCGAGACGAGA | GAAACCTCCCTCAGTGCCTC | Start: 650 |
| Tm | 59.965 | 60.036 | End: 826 |
| Size | 20 | 20 | 177 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATGTCGGTGCGGATGAAA | TCGTGTCCGTTTGAGACCTG | Start: 560 |
| Tm | 60.037 | 59.968 | End: 747 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
|---|
| Sequence | TGACGGCCCTACTCCATGTA | GAAACCTCCCTCAGTGCCTC | Start: 706 |
| Tm | 60.033 | 60.036 | End: 826 |
| Size | 20 | 20 | 121 |
| Primer 3 details |
|---|
| Sequence | TTGTCTTGTTGGGGGCTGTT | TTTCATCCGCACCGACATGA | Start: 376 |
| Tm | 60.033 | 60.037 | End: 579 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TCATGTCGGTGCGGATGAAA | TACATGGAGTAGGGCCGTCA | Start: 560 |
| Tm | 60.037 | 60.033 | End: 725 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | AGTGTCAGGTCGAGACGAGA | GAAACCTCCCTCAGTGCCTC | Start: 650 |
| Tm | 59.965 | 60.036 | End: 826 |
| Size | 20 | 20 | 177 |
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