Primer Details for TRINITY_DN83518_c4_g3_i1 |
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| (A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTACGCATGAATTCAGCT | TGAAGGAATGGAAAAGAAACATCAGT | Start: 26 |
| Tm | 57.972 | 59.449 | End: 220 |
| Size | 20 | 26 | 195 |
| Primer 2 details |
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| Sequence | TGCTACGCATGAATTCAGCT | GGTATCACCTCCATTACATACAAGT | Start: 26 |
| Tm | 57.972 | 58.172 | End: 190 |
| Size | 20 | 25 | 165 |
| Primer 3 details |
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| Sequence | TTGCTACGCATGAATTCAGCT | TGAAGGAATGGAAAAGAAACATCAGT | Start: 25 |
| Tm | 58.641 | 59.449 | End: 220 |
| Size | 21 | 26 | 196 |
| Primer 4 details |
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| Sequence | TGCTACGCATGAATTCAGCT | TGAAGGAATGGAAAAGAAACATCA | Start: 26 |
| Tm | 57.972 | 57.025 | End: 220 |
| Size | 20 | 24 | 195 |
| Primer 5 details |
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| Sequence | TGCTACGCATGAATTCAGCT | AAGGAATGGAAAAGAAACATCAGT | Start: 26 |
| Tm | 57.972 | 57.016 | End: 218 |
| Size | 20 | 24 | 193 |
